Over a hundred different bird species have been recorded around the creek, some 80 noted annually. From Great Egrets to Grebes, Mission Creek is a bird sanctuary in San Francisco. The Creek is also home to at least 10 butterfly species.
With the expansion of Huffaker Park to the south, the Conservancy is overseeing permanent bird perch piling installations in the Creek to provide safe perches for local water birds as well as make them more visible for creek visitors.
Mission Creek regularly hosts Bat Rays,
Sea Lions, Harbor Seals, anchovies, sardines, crabs and a rich forest of invertebrates and seaweeds growing in the tidal waters.